V8蛋白酶S
中文名稱：V8蛋白酶S
其他中文名稱：蛋白酶S
英文名稱： Protease S Aureus
其他英文名稱： Endoproteinase Glu-C from Staphylococcus aureus V8；V8 Protease
產(chǎn)品規(guī)格： BR，500u/mg
包裝： 5毫克/1毫克
CAS號：66676-43-5
級別：BR
來源：Staph aureus V8
Activity：≥500units/mg
V8蛋白酶S活力定義：One Unit causes a change of 0.001 A280 nm per minute at 37℃, pH 7.8 using casein as the substrate
產(chǎn)品描述：Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).
性狀：粉末。
用途：生化研究。
V8蛋白酶S保存：2～8℃